Novel synthetic analogs of sialyl Lewis X can inhibit angiogenesis in vitro and in vivo

被引：31

作者：

Nguyen, M ^{[1
]}

Eilber, FR ^{[1
]}

Defrees, S ^{[1
]}

机构：

[1] CYTEL CORP, LOS ANGELES, CA 90095 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 228卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.1722

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Selectins have been shown to play an important role in angiogenesis. We have set out to synthesize multiple analogs of the selectin ligand sialyl Lewis X. We show here that analogs of sialyl Lewis X at concentrations of 50 mu g/ml or greater significantly inhibited angiogenesis in in vitro assays of endothelial cell migration and proliferation (p < 0.05). These analogs also exerted significant inhibitory effects in in vivo angiogenesis assays using the chick chorioallantoic membrane at doses of 2 mg/chick or greater (p < 0.05). In contrast. the related carbohydrate Lewis X did nor inhibit angiogenesis in both in vitro and in vivo assays. These data indicate thar angiogenesis can be inhibited specifically by synthetic analogs of sialyl Lewis X. These analogs may potentially be useful in the treatment of angiogenesis-related diseases. (C) 1996 Academic Press.

引用

页码：716 / 723

页数：8

共 18 条

[1] SYNTHESIS AND STRUCTURAL-ANALYSIS USING 2-D NMR OF SIALYL LEWIS-X (SLE(X)) AND LEWIS-X (LE(X)) OLIGOSACCHARIDES - LIGANDS RELATED TO E-SELECTIN [ELAM-1] BINDING [J].