Simple diagnosis of Encephalitozoon sp microsporidial infections by using a panspecific antiexospore monoclonal antibody

被引:48
作者
Enriquez, FJ [1 ]
Ditrich, O [1 ]
Palting, JD [1 ]
Smith, K [1 ]
机构
[1] ACAD SCI CZECH REPUBL,INST PARASITOL,CR-37005 CESKE BUDEJOVICE,CZECH REPUBLIC
关键词
D O I
10.1128/JCM.35.3.724-729.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microsporidia (phylum Microspora) have recently become recognized as common opportunistic protozoans in the United States and worldwide, particularly affecting immunodeficient patients, Microsporidian organisms within the genus Encephalitozoon are the cause of nephrologic, ophthalmic, pneumologic, gastroenteric, and systemic infections, However, diagnosis of the small spores by light microscopy is difficult, even,vith newly developed and improved staining techniques, We have developed an anti-Encephnlitozoon species monoclonal antibody-based immunoassay for easy diagnosis, A hybridoma was produced and selected following one main criterion: recognition by immunofluorescence of all known Encephalitozoon spores affecting humans, The selected monoclonal antibody-secreting hybridomas were characterized by enzyme-linked immunosorbent assay, immunofluorescence, Western blot, and immunoelectron microscopy using Encephalitozoon species from fresh and fixed samples from patients and from in vitro cultures, In the immunofluorescence assay, one monoclonal antibody, termed 3B6, strongly recognized Encephalitozoon cuniculi, E, hellem, and E, intestinalis, Monoclonal antibody 3B6 bound to other microsporidia (Nosema and Vairimorpha spp,) without cross-reacting with any other parasite, including Enterocytozoon bieneusi, fungus, or bacterium tested, In immunoelectron microscopy assays, monoclonal antibody 3B6 bound to the exospore of Encephalitozoon species, while in Western blot assays, it recognized three to seven antigens with molecular masses ranging from 34 to 117 kDa, We have developed a sensitive and specific monoclonal antibody-based immunoassay to diagnose common microsporidian infections, particularly with Encephalitozoon species, This is a new tool for identifying spores in bodily fluids and biopsy samples and is an efficient diagnostic test, Additionally, monoclonal antibody 3B6 can serve to assess the prevalence of microsporidial infections in immunodeficient and immunocompetent patients.
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页码:724 / 729
页数:6
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