Homonuclear Hartmann-Hahn transfer with reduced relaxation losses by use of the MOCCA-XY16 multiple pulse sequence

被引:28
作者
Furrer, J
Kramer, F
Marino, JP
Glaser, SJ
Luy, B
机构
[1] Tech Univ Munich, Inst Organ Chem & Biochem, D-85747 Garching, Germany
[2] Ctr Adv Res Biotechnol, Rockville, MD 20878 USA
关键词
Hartmann-Hahn transfer; isotropic mixing; relaxation; TOCSY; invariant trajectory;
D O I
10.1016/j.jmr.2003.09.013
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Homonuclear Hartmann-Hahn transfer is one of the most important building blocks in modern high-resolution NMR. It constitutes a very efficient transfer element for the assignment of proteins, nucleic acids, and oligosaccharides. Nevertheless, in macromolecules exceeding similar to10 kDa TOCSY-experiments can show decreasing sensitivity due to fast transverse relaxation processes that are active during the mixing periods. In this article we propose the MOCCA-XY16 multiple pulse sequence, originally developed for efficient TOCSY transfer through residual dipolar couplings, as a homonuclear Hartmann-Hahn sequence with improved relaxation properties. A theoretical analysis of the coherence transfer via scalar couplings and its relaxation behavior as well as experimental transfer curves for MOCCA-XY16 relative to the well-characterized DIPSI-2 multiple pulse sequence are given. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:39 / 46
页数:8
相关论文
共 49 条
[1]  
BAX A, 1988, ISRAEL J CHEM, V28, P309
[2]   A THEORETICAL-ANALYSIS OF HOMONUCLEAR CROSS-POLARIZATION COHERENCE TRANSFER IN LIQUIDS [J].
BAZZO, R ;
BOYD, J .
JOURNAL OF MAGNETIC RESONANCE, 1987, 75 (03) :452-466
[3]   CHEMICAL-SHIFT CORRELATION SPECTROSCOPY IN ROTATING SOLIDS - RADIO FREQUENCY-DRIVEN DIPOLAR RECOUPLING AND LONGITUDINAL EXCHANGE [J].
BENNETT, AE ;
OK, JH ;
GRIFFIN, RG ;
VEGA, S .
JOURNAL OF CHEMICAL PHYSICS, 1992, 96 (11) :8624-8627
[4]   COHERENCE TRANSFER BY ISOTROPIC MIXING - APPLICATION TO PROTON CORRELATION SPECTROSCOPY [J].
BRAUNSCHWEILER, L ;
ERNST, RR .
JOURNAL OF MAGNETIC RESONANCE, 1983, 53 (03) :521-528
[5]   COMPUTER-OPTIMIZED HOMONUCLEAR TOCSY EXPERIMENTS WITH SUPPRESSION OF CROSS RELAXATION [J].
BRIAND, J ;
ERNST, RR .
CHEMICAL PHYSICS LETTERS, 1991, 185 (3-4) :276-285
[6]   COIN TACSY, a novel approach to tailored correlation spectroscopy [J].
Carlomagno, T ;
Prasch, T ;
Glaser, SNJ .
JOURNAL OF MAGNETIC RESONANCE, 2001, 149 (01) :52-57
[7]   EFFECTS OF DIFFUSION ON FREE PRECESSION IN NUCLEAR MAGNETIC RESONANCE EXPERIMENTS [J].
CARR, HY ;
PURCELL, EM .
PHYSICAL REVIEW, 1954, 94 (03) :630-638
[8]   SUPPRESSION OF CROSS-RELAXATION EFFECTS IN TOCSY SPECTRA VIA A MODIFIED DIPSI-2 MIXING SEQUENCE [J].
CAVANAGH, J ;
RANCE, M .
JOURNAL OF MAGNETIC RESONANCE, 1992, 96 (03) :670-678
[9]   ANALYSIS OF COLLECTIVE MODES IN SOME AMXN SYSTEMS [J].
CHANDRAKUMAR, N ;
VISALAKSHI, GV ;
RAMASWAMY, D ;
SUBRAMANIAN, S .
JOURNAL OF MAGNETIC RESONANCE, 1986, 67 (02) :307-318
[10]  
CHO HM, 1985, PHYS REV LETT, V56, P1905