How to detect Clostridium difficile variant strains in a routine laboratory

被引：55

作者：

Rupnik, M ^{[1
]}

机构：

[1] Univ Ljubljana, Dept Biol, Ljubljana 1000, Slovenia

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2001年 / 7卷 / 08期

关键词：

Clostridium difficle; detection; variant strains; toxinotypes; diagnostics;

D O I：

10.1046/j.1198-743x.2001.00290.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Toxin A-negative, toxin B-positive strains (A-/B+) are the best studied examples of Clostridium difficile variant strains. In addition, there are some other groups of variant C. difficile strains that produce both toxins (A+/B+) or are non-cytotoxic (A-/B-) but differ from the reference strain VPI 10463 in their toxin genes. Here we describe two simple methods (amplification of the tcdA gene and amplification of the binary toxin gene cdtA) which can be used in rapid screening for variant C. difficile strains in collections or in routine laboratories.

引用

收藏

页码：417 / 420

页数：4

相关论文

共 21 条

[1] Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile responsible for a nosocomial outbreak of Clostridium difficile-associated diarrhea [J].

Alfa, MJ ;

Kabani, A ;

Lyerly, D ;

Moncrief, S ;

Neville, LM ;

Al-Barrak, A ;

Harding, GKH ;

Dyck, B ;

Olekson, K ;

Embil, JM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) :2706-2714

[2]

BARBUT F, 2001, CLIN MICROBIOL INFEC, V7, P318

[3] DETECTION OF CLOSTRIDIUM-DIFFICILE ENTEROTOXIN GENE IN CLINICAL SPECIMENS BY THE POLYMERASE CHAIN-REACTION [J].

BOONDEEKHUN, HS ;

GURTLER, V ;

ODD, ML ;

WILSON, VA ;

MAYALL, BC .

JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 38 (05) :384-387

[4] MOLECULAR, IMMUNOLOGICAL, AND BIOLOGICAL CHARACTERIZATION OF A TOXIN-A-NEGATIVE, TOXIN-B-POSITIVE STRAIN OF CLOSTRIDIUM-DIFFICILE [J].

BORRIELLO, SP ;

WREN, BW ;

HYDE, S ;

SEDDON, SV ;

SIBBONS, P ;

KRISHNA, MM ;

TABAQCHALI, S ;

MANEK, S ;

PRICE, AB .

INFECTION AND IMMUNITY, 1992, 60 (10) :4192-4199

[5] Detection of the ADP-ribosyltransferase toxin gene (cdtA) and its activity in Clostridium difficile isolates from Equidae [J].

Braun, M ;

Herholz, C ;

Straub, R ;

Choisat, B ;

Frey, J ;

Nicolet, J ;

Kuhnert, P .

FEMS MICROBIOLOGY LETTERS, 2000, 184 (01) :29-33

[6] The diagnosis of Clostridium difficile-associated disease [J].

Brazier, JS .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1998, 41 :29-40

[7] A novel cytotoxin from Clostridium difficile serogroup F is a functional hybrid between two other large clostridial cytotoxins [J].

Chaves-Olarte, E ;

Löw, P ;

Freer, E ;

Norlin, T ;

Weidmann, M ;

von Eichel-Streiber, C ;

Thelestam, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (16) :11046-11052

[8] SEROGROUP-F STRAINS OF CLOSTRIDIUM-DIFFICILE PRODUCE TOXIN-B BUT NOT TOXIN-A [J].

DEPITRE, C ;

DELMEE, M ;

AVESANI, V ;

LHARIDON, R ;

ROELS, A ;

POPOFF, M ;

CORTHIER, G .

JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 38 (06) :434-441

[9]

EICHELSTREIBER CV, 1995, MOL MICROBIOL, V17, P313

[10] Identification of toxin A-negative, toxin B-positive Clostridium difficile by PCR [J].

Kato, H ;

Kato, N ;

Watanabe, K ;

Iwai, N ;

Nakamura, H ;

Yamamoto, T ;

Suzuki, K ;

Kim, SM ;

Chong, Y ;

Wasito, EB .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (08) :2178-2182