Development of microsatellite markers in bonytail (Gila elegans) with cross-species amplification in humpback chub (Gila cypha)

被引：9

作者：

Keeler-Foster, CL

Spies, IB

Bondu-Hawkins, V

Bentzen, P

机构：

[1] Dexter NFHTC, Mol Ecol Program, Dexter, NM 88230 USA

[2] Alaska Fisheries Sci Ctr, Seattle, WA 98115 USA

[3] Univ New Mexico, Dept Pathol, Infect Dis & Inflammat Program, Albuquerque, NM 87131 USA

[4] Dalhousie Univ, Dept Biol, Fisheries Resource Conservat Genet, Halifax, NS B3H 4J1, Canada

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 01期

关键词：

bonytail; Dexter National Fish Hatchery and Technology Center; enrichment; Gila; humpback; microsatellites;

D O I：

10.1046/j.1471-8286.2003.00553.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bonytail, Gila elegans, is an endangered species of fish native to the Colorado River. Primers are presented for 17 microsatellites cloned from bonytail as well as the results of test amplifications in bonytail and humpback chub, G. cypha. Bonytail exhibited three to 18 alleles per locus across the 17 microsatellites and a mean expected heterozygosity of 0.58 among 10 loci used to screen 160 broodstock. Humpback chub exhibited one to six alleles and a mean expected heterozygosity of 0.69 among 10 loci that were successfully amplified in that species.

引用

页码：23 / 25

页数：3