Fruit-specific RNAi-mediated suppression of SlNCED1 increases both lycopene and β-carotene contents in tomato fruit

被引:149
作者
Sun, Liang [1 ]
Yuan, Bing [2 ]
Zhang, Mei [3 ]
Wang, Ling [1 ]
Cui, Mengmeng [1 ]
Wang, Qi [1 ]
Leng, Ping [1 ]
机构
[1] China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China
[2] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[3] Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China
关键词
ABA; beta-carotene; carotenoid biosynthesis; ethylene; fruit ripening; lycopene; SlNCED1-RNAi; tomato; ABSCISIC-ACID BIOSYNTHESIS; LYCOPERSICON-ESCULENTUM; ZEAXANTHIN EPOXIDASE; ETHYLENE PRODUCTION; FLAVONOID CONTENT; PLANT DEVELOPMENT; ANTISENSE RNA; EXPRESSION; PROMOTER; ABA;
D O I
10.1093/jxb/ers026
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Abscisic acid (ABA) plays important roles during tomato fruit ripening. To study the regulation of carotenoid biosynthesis by ABA, the SlNCED1 gene encoding 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the ABA biosynthesis, was suppressed in tomato plants by transformation with an RNA interference (RNAi) construct driven by a fruit-specific E8 promoter. ABA accumulation and SlNCED1 transcript levels in the transgenic fruit were down-regulated to between 20-50% of that in control fruit. This significant reduction in NCED activity led to the carbon that normally channels to free ABA as well as the ABA metabolite accumulation during ripening to be partially blocked. Therefore, this 'backlogged' carbon transformed into the carotenoid pathway in the RNAi lines resulted in increased assimilation and accumulation of upstream compounds in the pathway, chiefly lycopene and beta-carotene. Fruit of all RNAi lines displayed deep red coloration compared with the pink colour of control fruit. The decrease in endogenous ABA in these transgenics resulted in an increase in ethylene, by increasing the transcription of genes related to the synthesis of ethylene during ripening. In conclusion, ABA potentially regulated the degree of pigmentation and carotenoid composition during ripening and could control, at least in part, ethylene production and action in climacteric tomato fruit.
引用
收藏
页码:3097 / 3108
页数:12
相关论文
共 47 条
[1]   Signal transduction systems regulating fruit ripening [J].
Adams-Phillips, L ;
Barry, C ;
Giovannoni, J .
TRENDS IN PLANT SCIENCE, 2004, 9 (07) :331-338
[2]   High-flavonol tomatoes resulting from the heterologous expression of the maize transcription factor genes LC and C1 [J].
Bovy, A ;
de Vos, R ;
Kemper, M ;
Schijlen, E ;
Pertejo, MA ;
Muir, S ;
Collins, G ;
Robinson, S ;
Verhoeyen, M ;
Hughes, S ;
Santos-Buelga, C ;
van Tunen, A .
PLANT CELL, 2002, 14 (10) :2509-2526
[3]   Sesquiterpene-like inhibitors of a 9-cis-epoxycarotenoid dioxygenase regulating abscisic acid biosynthesis in higher plants [J].
Boyd, Jason ;
Gai, Yuanzhu ;
Nelson, Ken M. ;
Lukiwski, Erica ;
Talbot, James ;
Loewen, Mary K. ;
Owen, Stacey ;
Zaharia, L. Irina ;
Cutler, Adrian J. ;
Abrams, Suzanne R. ;
Loewen, Michele C. .
BIOORGANIC & MEDICINAL CHEMISTRY, 2009, 17 (07) :2902-2912
[4]   BIOCHEMICAL-CHARACTERIZATION OF TRANSGENIC TOMATO PLANTS IN WHICH CAROTENOID SYNTHESIS HAS BEEN INHIBITED THROUGH THE EXPRESSION OF ANTISENSE RNA TO PTOM5 [J].
BRAMLEY, P ;
TEULIERES, C ;
BLAIN, I ;
BIRD, C ;
SCHUCH, W .
PLANT JOURNAL, 1992, 2 (03) :343-349
[5]  
BUESA C, 1994, REV ESP CIEN TEC ALI, V34, P495
[6]   Structure and expression of a cDNA encoding zeaxanthin epoxidase, isolated from a wilt-related tomato (Lycopersicon esculentum Mill.) library [J].
Burbidge, A ;
Grieve, T ;
Terry, C ;
Corlett, J ;
Thompson, A ;
Taylor, I .
JOURNAL OF EXPERIMENTAL BOTANY, 1997, 48 (314) :1749-1750
[7]   Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors [J].
Butelli, Eugenio ;
Titta, Lucilla ;
Giorgio, Marco ;
Mock, Hans-Peter ;
Matros, Andrea ;
Peterek, Silke ;
Schijlen, Elio G. W. M. ;
Hall, Robert D. ;
Bovy, Arnaud G. ;
Luo, Jie ;
Martin, Cathie .
NATURE BIOTECHNOLOGY, 2008, 26 (11) :1301-1308
[8]   Regulation of a carotenoid biosynthesis gene promoter during plant development [J].
Corona, V ;
Aracri, B ;
Kosturkova, G ;
Bartley, GE ;
Pitto, L ;
Giorgetti, L ;
Scolnik, PA ;
Giuliano, G .
PLANT JOURNAL, 1996, 9 (04) :505-512
[9]   Quantitative analysis of the flavonoid content of commercial tomatoes, onions, lettuce, and celery [J].
Crozier, A ;
Lean, MEJ ;
McDonald, MS ;
Black, C .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (03) :590-595
[10]   Genes and enzymes of carotenoid biosynthesis in plants [J].
Cunningham, FX ;
Gantt, E .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1998, 49 :557-583