Evaluation and application of conducting polymer entrapment on quartz crystal microbalance in flow injection immunoassay

被引：22

作者：

Liu, YC ^{[1
]}

Wang, CM

Hsiung, KP

Huang, CJ

机构：

[1] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 402, Taiwan

[2] Ind Technol Res Inst, Ctr Biomed Engn, Hsinchu 300, Taiwan

[3] Natl Chung Hsing Univ, Grad Inst Vet Microbiol, Taichung 402, Taiwan

来源：

BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 07期

关键词：

quartz crystal microbalance; flow-injection-analysis; conducting polymer entrapment; pseudorabies virus; point-of-care testing;

D O I：

10.1016/S0956-5663(02)00208-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An immunosensor employing conducting polymer entrapment (CPE) method to immobilize immuno-protein on the quartz crystal microbalance (QCM) for clinical flow-injection-analysis (FIA) purpose was exploited. By comparing the CPE approach and the conventional physical immobilization (PI) method, a frequency shift of the former was 47% higher than that of the latter when measuring at 0.5 mg/ml human serum albumin antibody concentration. This implied that CPE was a feasible approach for developing QCM-FIA process. An immunoassay of anti-pseudorabies virus antibody in mouse sera further exemplified its practical potential in diagnostic implication. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：937 / 942

页数：6

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