tRNA modification profileq of the fast proliferating cancer cells

被引：41

作者：

Dong, Chao ^{[1
]}

Niu, Leilei ^{[1
]}

Song, Wei ^{[1
]}

Xiong, Xin ^{[2
]}

Zhang, Xianhua ^{[2
]}

Zhang, Zhenxi ^{[1
]}

Yang, Yi ^{[1
]}

Yi, Fan ^{[1
]}

Zhan, Jun ^{[3
]}

Zhang, Hongquan ^{[3
]}

Yang, Zhenjun ^{[1
]}

Zhang, Li-He ^{[1
]}

Zhai, Suodi ^{[2
]}

Li, Hua ^{[1
]}

Ye, Min ^{[1
]}

Du, Quan ^{[1
]}

机构：

[1] Peking Univ, Hosp 3, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci,Dept Obstet & Gynecol, Beijing 100191, Peoples R China

[2] Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China

[3] Peking Univ, Dept Anat Histol & Embryol, Lab Mol Cell Biol & Tumor Biol, Beijing 100191, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 476卷 / 04期

基金：

北京市自然科学基金;

关键词：

RNA modification; Cancer; Proliferation; DYNAMICS;

D O I：

10.1016/j.bbrc.2016.05.124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Despite the recent progress in RNA modification study, a comprehensive modification profile is still lacking for mammalian cells. Using a quantitative HPLC/MS/MS assay, we present here a study where RNA modifications are examined in term of the major RNA species. With paired slow- and fast proliferating cell lines, distinct RNA modification profiles are first revealed for diverse RNA species. Compared to mRNAs, increased ribose and nucleobase modifications are shown for the highly-structured tRNAs and rRNAs, lending support to their contribution to the formation of high-order structures. This study also reveals a dynamic tRNA modification profile in the fast-proliferating cells. In addition to cultured cells, this unique tRNA profile has been further confirmed with endometrial cancers and their adjacent normal tissues. Taken together, the results indicate that tRNA is a actively regulated RNA species in the fast-proliferating cancer cells, and suggest that they may play a more active role in biological process than expected. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：340 / 345

页数：6

共 15 条

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