Gram type-specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria

被引：59

作者：

Klausegger, A

Hell, M

Berger, A

Zinober, K

Baier, S

Jones, N

Sperl, W

Kofler, B

机构：

[1] Gen Hosp Salzburg, Childrens Hosp, A-5020 Salzburg, Austria

[2] Gen Hosp Salzburg, Inst Bacteriol, Salzburg, Austria

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 02期

关键词：

D O I：

10.1128/JCM.37.2.464-466.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either grampositive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.

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收藏

页码：464 / 466

页数：3

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