Simple and reliable method to precipitate proteins from bacterial culture supernatant

被引：31

作者：

Caldwell, RB ^{[1
]}

Lattemann, CT ^{[1
]}

机构：

[1] CREATOGEN AG, D-86156 Augsburg, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 01期

关键词：

D O I：

10.1128/AEM.70.1.610-612.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.

引用

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页码：610 / 612

页数：3

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