High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells

被引：48

作者：

Gutierrez, Edgar ^{[1
]}

Tkachenko, Eugene ^{[1
,2
]}

Besser, Achim ^{[3
]}

Sundd, Prithu ^{[4
]}

Ley, Klaus ^{[4
]}

Danuser, Gaudenz ^{[3
]}

Ginsberg, Mark H. ^{[2
]}

Groisman, Alex ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Phys, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA

[3] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA USA

[4] La Jolla Inst Allergy & Immunol, Div Inflammat Biol, La Jolla, CA USA

来源：

PLOS ONE | 2011年 / 6卷 / 09期

基金：

美国国家卫生研究院;

关键词：

SUBSTRATE STIFFNESS; FOCAL ADHESIONS; LOCOMOTION; MATRICES; GROWTH; TIRF;

D O I：

10.1371/journal.pone.0023807

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate.

引用

页数：5

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