Gephyrin-independent clustering of postsynaptic GABAA receptor subtypes

被引:124
作者
Kneussel, M
Brandstätter, JH
Gasnier, B
Feng, GP
Sanes, JR
Betz, H
机构
[1] Max Planck Inst Brain Res, Dept Neurochem, D-60528 Frankfurt, Germany
[2] Max Planck Inst Brain Res, Dept Neuroanat, D-60528 Frankfurt, Germany
[3] CNRS, Inst Biol Physicochim, UPR 6 1929, F-75005 Paris, France
[4] Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA
关键词
D O I
10.1006/mcne.2001.0983
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Gephyrin has been shown to be essential for the synaptic localization of the inhibitory glycine receptor and major GABA(A) receptor (GABA(A)R) subtypes. However, in retina certain GABA(A)R subunits are found at synaptic sites in the absence of gephyrin. Here, we quantitatively analyzed GABA(A)R alpha1, alpha2, alpha3, alpha5, beta2/3, and gamma2 subunit immunoreactivities in spinal cord sections derived from wild-type and gephyrin-deficient (geph -/-) mice. The punctate staining of GABA(A)R alpha1 and alpha5 subunits was unaltered in geph -/- mice, whereas the numbers of alpha2-, alpha3-, beta2/3-, and gamma2-subunit-immunoreactive synaptic sites were significantly or even strikingly reduced in the mutant animals. Immunostaining with an antibody specific for the vesicular inhibitory amino acid transporter revealed that the number of inhibitory presynaptic terminals is unaltered upon gephyrin deficiency. These data show that in addition to gephyrin other clustering proteins must exist that mediate the synaptic localization of selected GABA(A)R subtypes.
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收藏
页码:973 / 982
页数:10
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