A high pressure matrix-assisted laser desorption ion source for Fourier transform mass spectrometry designed to accommodate large targets with diverse surfaces

被引：49

作者：

O'Connor, PB ^{[1
]}

Budnik, BA ^{[1
]}

Ivleva, VB ^{[1
]}

Kaur, P ^{[1
]}

Moyer, SC ^{[1
]}

Pittman, JL ^{[1
]}

Costello, CE ^{[1
]}

机构：

[1] Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2004年 / 15卷 / 01期

关键词：

D O I：

10.1016/j.jasms.2003.10.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new design for a high pressure MALDI-FTMS instrument is described and initial data are shown. The instrument incorporates a large, 10 cm x 10 cm, sample translation stage to accommodate and position the MALDI target. The new instrument allows coupling to a wide variety of surface techniques such as gel electrophoresis or surface plasmon resonance. Coupling to thin layer chromatography is shown. Furthermore, a new nozzle design allows high pressure collisional cooling sufficient to stabilize gangliosides while minimizing the gas load on the system.

引用

页码：128 / 132

页数：5

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