ORF90, a gene required for photoreactivation in Rhodobacter capsulatus SB1003 encodes a cyclobutane pyrimidine dimer photolyase

被引:4
作者
Braatsch, S [1 ]
Klug, G [1 ]
机构
[1] Univ Giessen, Inst Mikrobiol & Mol Biol, D-35392 Giessen, Germany
关键词
cryptochrome/photolyase family; photolyase; photoreactivation; Rhodobacter;
D O I
10.1023/B:PRES.0000015396.99271.90
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Based on deduced amino-acid sequence similarities to class-I photolyases, the open reading frame ORF90 was identified from the genome sequence of Rhodobacter capsulatus SB1003. Photoreactivation activity is not detectable in an ORF90 deletion mutant of R. capsulatus SB1003. The phenotype of R. capsulatus wild-type cells was restored by plasmid borne ORF90 of R. capsulatus DeltaORF90. Furthermore, we detected an ORF90-related CPD-specific photoreactivation activity in R. capsulatus cell extracts. The results show that the gene product of ORF90 is involved in photoreactivation and encodes a class-I cyclobutane pyrimidine dimer photolyase.
引用
收藏
页码:167 / 177
页数:11
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