A droplet-based lab-on-a-chip for colorimetric detection of nitroaromatic explosives

被引：22

作者：

Pamula, VK ^{[1
]}

Srinivasan, V ^{[1
]}

Chakrapani, H ^{[1
]}

Fair, RB ^{[1
]}

Toone, EJ ^{[1
]}

机构：

[1] Duke Univ, Dept Elect Engn, Durham, NC 27708 USA

来源：

MEMS 2005 Miami: Technical Digest | 2005年

关键词：

D O I：

10.1109/MEMSYS.2005.1454031

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Portable and automated field screening equipment would be very effective in detecting and quantifying explosives at various sites. A droplet-based microfluidic lab-on-a-chip utilizing electrowetting is presented for the colorimetric detection of TNT (trinitrotoluene). The method uses the reaction between nitroaromatics and a strong base which forms the highly colored Jackson-Meisenheimer complex. Microliter-sized droplets of TNT are programmed to transport, mix, and react with potassium hydroxide (KOH) on the microfluidic chip. Colorimetric reactions of TNT are characterized both on a spectrophotometer and on the microfluidic chip. The detection of TNT on the chip is linear in the range of 4-20 mu g/mL with a time-to-result of 2.5minutes. It is also observed that the absorbance peaks of DNT (dinitrotoluene) and TNT are mutually independent and that the presence of DNT does not affect the detection of TNT. Electrowetting also does not seem to influence the colorimetric complex as observed from a comparison of results between a spectrophotometer and on-chip.

引用

页码：722 / 725

页数：4

共 11 条

[1]

CRAIG H, 1996, P HSRC WERC JOINT C

[2]

CROCKETT AB, 1996, EPA549R97501

[3] DEVELOPMENT OF FIELD SCREENING METHODS FOR TNT, 2,4-DNT AND RDX IN SOIL [J].