Decreased expression of topoisomerase IIβ in poly(ADP-ribose) polymerase-deficient cells

被引：10

作者：

Canitrot, Y

de Murcia, G

Salles, B

机构：

[1] CNRS, UPR 9062, Inst Pharmacol & Biol Struct, F-31077 Toulouse, France

[2] Ecole Super Biotechnol Strasbourg, CNRS, UPR 9003, F-67400 Illkirch, France

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 22期

关键词：

D O I：

10.1093/nar/26.22.5134

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies with poly(ADP-ribose) polymerase (PARP)-deficient mice have highlighted the role of this enzyme in genomic stability and response to various genomic insults. In the absence of DNA damaging treatment, we report here that a PARP-deficient cell line (PARP-/-) established from knockout mice displays a decrease in topoisomerase II (topo II) activity as measured by decatenation of kinetoplast DNA, Immunoblotting of whole and nuclear cell extracts showed that reduced activity was associated with decreased amount of the 180 kDa topo II beta protein but not of the 170 kDa topo II alpha. The decreased topo II beta expression did not stem from transcriptional regulation of gene expression since levels of topo II beta mRNA were similar in PARR (-/-) compared with the parental PARP (+/+) cells. The decreased topo II activity was associated with cell resistance to VP16, a topo II inhibitor. These observations indicate that PARP may play a role in the stabilization and/or distribution of topo II beta.

引用

页码：5134 / 5138

页数：5

共 34 条

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