Evaluating cutaneous photoaging by use of multiphoton fluorescence and second-harmonic generation microscopy

被引：199

作者：

Lin, SJ ^{[1
]}

Wu, RJ

Tan, HY

Lo, W

Lin, WC

Young, TH

Hsu, CJ

Chen, JS

Jee, SH

Dong, CY

机构：

[1] Natl Taiwan Univ Hosp, Taipei, Taiwan

[2] Natl Taiwan Univ, Dept Phys, Taipei, Taiwan

[3] Natl Taiwan Univ, Inst Biomed Engn, Taipei 10764, Taiwan

[4] Chang Gung Mem Hosp, Dept Ophthalmol, Linkou, Taiwan

[5] Natl Taiwan Univ Hosp, Dept Pathol, Taipei 100, Taiwan

[6] Natl Taiwan Univ, Inst Biomed Engn, Taipei, Taiwan

[7] Natl Taiwan Univ Hosp, Dept Dermatol, Taipei, Taiwan

[8] Natl Taiwan Univ, Coll Med, Taipei, Taiwan

[9] Natl Taiwan Univ Hosp, Yun Lin Branch, Yunlin, Taiwan

来源：

OPTICS LETTERS | 2005年 / 30卷 / 17期

关键词：

D O I：

10.1364/OL.30.002275

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The photoaging process of facial skin is investigated by use of multiphoton fluorescence and second-harmonic generation (SHG) microscopy. We obtain the autofluorescence (AF) and SHG images of the superficial dermis from the facial skin of three patients aged 20, 40, and 70 years. The results show that areas of AF increase with age, whereas areas of SHG decrease with age. The results are consistent with the histological findings in which collagen is progressively replaced by elastic fibers. The AF and SHG changes in photoaging are quantified by a SHG to autofluorescence aging index of dermis (SAAID). Our results suggest that SAAID can be a good indicator of the severity of photoaging. (c) 2005 Optical Society of America.

引用

页码：2275 / 2277

页数：3

共 13 条

[1]

[Anonymous], 2003, Fitzpatrick's Dermatology in General Medicine

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