Spongy gel-like layered double hydroxide-alkaline phosphatase nanohybrid as a biosensing material

被引：48

作者：

Geraud, Erwan ^{[1
]}

Prevot, Vanessa ^{[1
]}

Forano, Claude ^{[1
]}

Mousty, Christine ^{[1
,2
]}

机构：

[1] Univ Blaise Pascal, Lab Mat Inorgan, CNRS, UMR 6002,FR 2404, F-63177 Aubiere, France

[2] Univ Grenoble 1, Dept Chim Mol, CNRS, UMR 5250,ICMG FR 2607, F-38041 Grenoble 9, France

来源：

CHEMICAL COMMUNICATIONS | 2008年 / 13期

关键词：

D O I：

10.1039/b715385f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Formation of new bio-nanohybrid material was obtained by immobilization of alkaline phosphatase within a Mg2Al LDH by "soft chemistry'' coprecipitation synthesis, resulting in an original spongy gel-like morphology allowing the preservation of the enzyme structure and activity even at low pH values thanks to the buffering property of the basic host structure.

引用

页码：1554 / 1556

页数：3

共 12 条

[1] Construction of an alkaline phosphatase-liposome system: a tool for biomineralization study [J].

Camolezi, FL ;

Daghastanli, KRP ;

Magalhaes, PP ;

Pizauro, JM ;

Ciancaglini, P .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2002, 34 (09) :1091-1101

[2] An bienzyme electrode (alkaline phosphatase polyphenol oxidase) for the amperometric determination of phosphate [J].

Cosnier, S ;

Gondran, C ;

Watelet, JC ;

De Giovani, W ;

Furriel, RPM ;

Leone, FA .

ANALYTICAL CHEMISTRY, 1998, 70 (18) :3952-3956

[3]

Forano C, 2006, CURR NANOSCI, V2, P283

[4] Sol-gel materials as efficient enzyme protectors: Preserving the activity of phosphatases under extreme pH conditions [J].

Frenkel-Mullerad, H ;

Avnir, D .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (22) :8077-8081

[5] Catalytic properties of layered double hydroxides and their calcined derivatives [J].

Kagunya, W ;

Hassan, Z ;

Jones, W .

INORGANIC CHEMISTRY, 1996, 35 (21) :5970-5974

[6] Sensors and biosensors based on clay-modified electrodes - new trends [J].

Mousty, C .

APPLIED CLAY SCIENCE, 2004, 27 (3-4) :159-177

[7] ANION-EXCHANGING CLAY-MODIFIED ELECTRODES - SYNTHETIC LAYERED DOUBLE HYDROXIDES INTERCALATED WITH ELECTROACTIVE ORGANIC-ANIONS [J].

MOUSTY, C ;

THERIAS, S ;

FORANO, C ;

BESSE, JP .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1994, 374 (1-2) :63-69

[8] Biosensors as useful tools for environmental analysis and monitoring [J].

Rodriguez-Mozaz, Sara ;

Lopez de Alda, Maria J. ;

Barcelo, Damia .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 386 (04) :1025-1041

[9] Recent advances in biosensor techniques for environmental monitoring [J].

Rogers, K. R. .

ANALYTICA CHIMICA ACTA, 2006, 568 (1-2) :222-231

[10] Activity and stability of alkaline phosphatase (ALP) immobilized onto magnetic nanoparticles (Fe3O4) [J].

Saiyed, Z. M. ;

Sharma, S. ;

Godawat, R. ;

Telang, S. D. ;

Ramchand, C. N. .

JOURNAL OF BIOTECHNOLOGY, 2007, 131 (03) :240-244

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