Methodological aspects about in vitro evaluation of antioxidant properties

被引：568

作者：

Magalhaes, Luis M. ^{[1
]}

Segundo, Marcela A. ^{[1
]}

Reis, Salette ^{[1
]}

Lima, Jose L. F. C. ^{[1
]}

机构：

[1] Univ Porto, Fac Farm, Serv Quim Fis, REQUIMTE, P-4099030 Oporto, Portugal

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 613卷 / 01期

关键词：

antioxidants; scavenging capacity; reactive oxygen species; reactive nitrogen species;

D O I：

10.1016/j.aca.2008.02.047

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Several of the most commonly used methods for in vitro determination of antioxidant capacity are reviewed in the present paper. The chemical principles of methods based either on biological oxidants (peroxyl radical, superoxide radical anion, hydrogen peroxide, hydroxyl radical, hypochlorous acid, singlet oxygen, nitric oxide radical, and peroxynitrite) or on non-biological assays (scavenging of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (TEAC assay), scavenging of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH degrees assay), ferric reducing antioxidant power (FRAP assay), Folin-Ciocalteu reducing capacity (FC assay), electrochemical total reducing capacity) are outlined and critically discussed. The scope of application, the advantages and shortcomings of each method are also highlighted. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1 / 19

页数：19

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