Investigation of glucocorticoid-induced apoptotic pathway: Processing of Caspase-6 but not Caspase-3

被引:55
作者
Miyashita, T
Nagao, K
Krajewski, S
Salvesen, GS
Reed, JC
Inoue, T
Yamada, M
机构
[1] Natl Childrens Med Res Ctr, Dept Genet, Setagaya Ku, Tokyo 154, Japan
[2] Nihon Univ, Coll Biol Resource Sci, Fujisawa, Kanagawa 252, Japan
[3] Burnham Inst, La Jolla, CA 92037 USA
关键词
apoptosis; glucocorticoid; Bcl-2; caspase family;
D O I
10.1038/sj.cdd.4400442
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Glucocorticoids (GCs) are essential therapeutic reagents for the treatment of lymphomas and leukemias. GCs cause cell death in certain types of lymphoid cells mediated by the process known as apoptosis. This cell death is completely inhibited by Bcl-2, Here we report that Bcl-2 and benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (zVAD-fmk), a broad spectrum caspase inhibitor, prevent loss of mitochondrial membrane potential (Delta Psi m) and the production of reactive oxygen species (ROS) caused by GC, while acetyl-Asp-Glu-Val-Asp-aldehyde (Ac-DEVD-CHO), an inhibitor of the caspase-3 family proteases, does not. This suggests that the inhibition by Bcl-2 and activation of some initiator caspases are upstream events of mitochondrial damage, whereas the activation of caspase-3 family proteases occurs downstream of mitochondrial changes. We also demonstrate that caspase-6 but not caspase-3 is cleaved and activated during GC-mediated apoptosis and that poly(ADP-ribose) polymerase (PARP), a substrate of caspases, also undergoes proteolysis. in addition, we provide the evidence that DNA fragmentation is markedly inhibited by Ac-DEVD-CHO, while cell death, assessed by the damage of the plasma membrane, is marginally inhibited or merely delayed.
引用
收藏
页码:1034 / 1041
页数:8
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