Doxorubicin-loaded human serum albumin nanoparticles surface-modified with TNF-related apoptosis-inducing ligand and transferrin for targeting multiple tumor types

被引:227
作者
Bae, Sungho [2 ]
Ma, Kyungwan [2 ]
Kim, Tae Hyung [1 ]
Lee, Eun Seong [3 ]
Oh, Kyung Taek [4 ]
Park, Eun-Seok [1 ]
Lee, Kang Choon [1 ]
Youn, Yu Seok [1 ]
机构
[1] Sungkyunkwan Univ, Sch Pharm, Suwon 440746, South Korea
[2] Pusan Natl Univ, Coll Pharm, Pusan 609735, South Korea
[3] Catholic Univ Korea, Div Biotechnol, Bucheon Si 420743, Gyeonggi Do, South Korea
[4] Chung Ang Univ, Coll Pharm, Seoul 155756, South Korea
基金
新加坡国家研究基金会;
关键词
TRAIL; Albumin nanoparticles; Apoptosis; Doxorubicin; Tumor targeting; NECROSIS-FACTOR-ALPHA; DEATH RECEPTORS; CANCER-CELLS; APO-2; LIGAND; RESISTANCE; STABILITY; EXPRESSION; EFFICACY; SYSTEMS; TRAIL;
D O I
10.1016/j.biomaterials.2011.10.050
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Human serum albumin (HSA) nanoparticles (NPs) surface modified with tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and transferrin, and containing doxorubicin were designed and prepared. Surface amines of HSA were reversibly protected with dimethylmaleic anhydride (DMMA), and HSA-NPs were prepared using a desolvation technique. Furthermore, the surfaces of HSA-NPs were modified with thiolated TRAIL or transferrin using sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC). The prepared TRAIL/transferrin plus doxorubicin HSA-NPs were characterized by TEM, FE-SEM, and particle size analysis, and their cytotoxic and apoptotic activities were evaluated in several cancer cell lines, namely, HCT 116, doxorubicin-resistant MCF-7, and CAPAN-1. In addition, the tumor-targeting abilities of NPs were assessed using an infrared imaging system in HCT 116-xenografted nu/nu mice. Results showed that the TRAIL/transferrin/doxorubicin HSA-NPs had remarkable cytotoxic and apoptotic activities in all cancer cells examined with a general or a drug-resistant character, and that these NPs had obvious synergistic cytotoxic effects particularly on CAPAN-1 cells. Moreover, these HSA-NPs were effectively localized to tumors in a HCT 116-xenografted nu/nu mouse over 32 h. The findings of this study suggest that the described TRAIL/transferrin/doxorubicin HSA-NPs are a useful targeting agent capable of killing different types of tumor cells in various tissue organs. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1536 / 1546
页数:11
相关论文
共 43 条
[1]
Ligand-targeted therapeutics in anticancer therapy [J].
Allen, TM .
NATURE REVIEWS CANCER, 2002, 2 (10) :750-763
[2]
Apo2L/TRAIL: apoptosis signaling, biology, and potential for cancer therapy [J].
Almasan, A ;
Ashkenazi, A .
CYTOKINE & GROWTH FACTOR REVIEWS, 2003, 14 (3-4) :337-348
[3]
Transferrin-containing, cyclodextrin polymer-based particles for tumor-targeted gene delivery [J].
Bellocq, NC ;
Pun, SH ;
Jensen, GS ;
Davis, ME .
BIOCONJUGATE CHEMISTRY, 2003, 14 (06) :1122-1132
[4]
Doxorubicin-loaded PLGA nanoparticles by nanoprecipitation:: preparation, characterization and in vitro evaluation [J].
Betancourt, Tania ;
Brown, Brandon ;
Brannon-Peppas, Lisa .
NANOMEDICINE, 2007, 2 (02) :219-232
[5]
Active targeting schemes for nanoparticle systems in cancer therapeutics [J].
Byrne, James D. ;
Betancourt, Tania ;
Brannon-Peppas, Lisa .
ADVANCED DRUG DELIVERY REVIEWS, 2008, 60 (15) :1615-1626
[6]
Improved Antitumor Activity and Tumor Targeting of NH2-Terminal-Specific PEGylated Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand [J].
Chae, Su Young ;
Kim, Tae Hyung ;
Park, Kyeongsoon ;
Jin, Cheng-Hao ;
Son, Sohee ;
Lee, Seulki ;
Youn, Yu Seok ;
Kim, Kwangmeyung ;
Jo, Dong-Gyu ;
Kwon, Ick Chan ;
Chen, Xiaoyuan ;
Lee, Kang Choon .
MOLECULAR CANCER THERAPEUTICS, 2010, 9 (06) :1719-1729
[7]
Multifunctional Nanoparticles Delivering Small Interfering RNA and Doxorubicin Overcome Drug Resistance in Cancer [J].
Chen, Yunching ;
Bathula, Surendar Reddy ;
Li, Jun ;
Huang, Leaf .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (29) :22639-22650
[8]
Preparation, characterisation and maintenance of drug efficacy of doxorubicin-loaded human serum albumin (HSA) nanoparticles [J].
Dreis, S. ;
Rothweller, F. ;
Michaelis, A. ;
Cinatl, J., Jr. ;
Kreuter, J. ;
Langer, K. .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2007, 341 (1-2) :207-214
[9]
Polymer conjugates as anticancer nanomedicines [J].
Duncan, Ruth .
NATURE REVIEWS CANCER, 2006, 6 (09) :688-701
[10]
Doxorubicin increases the effectiveness of Apo2L/TRAIL for tumor growth inhibition of prostate cancer xenografts [J].
El-Zawahry, A ;
McKillop, J ;
Voelkel-Johnson, C .
BMC CANCER, 2005, 5 (1)