Direct electrogenerated chemiluminescence detection in high-performance liquid chromatography for determination of ofloxacin

被引：14

作者：

Sun, Yonghua ^{[1
]}

Zhang, Zhujun ^{[1
]}

Xi, Zhijun ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Coll Chem & Mat Sci, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 623卷 / 01期

基金：

中国国家自然科学基金;

关键词：

direct electrogenerated chemiluminescence; liquid chromatography; dual-electrode system; ofloxacin;

D O I：

10.1016/j.aca.2008.06.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ofloxacin (OFLX) exhibited strong electrogenerated chemiluminescence (ECL) in NaNO3 solution with a dual-electrode system when constant current was exerted. Based on this observation, a sensitive direct ECL method coupled with high-performance liquid chromatography (HPLC) separation was developed for determination of OFLX in human serum. Factors affected the ECL emission were investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of OFLX in the range of 1.0 x 10(-8) to 4.0 x 10(-6) g mL(-1) and the detection limit was 4 x 10(-9) g mL(-1) (S/N = 3). The proposed method was sensitive, simple and convenient to operate. (C) 2008 Elsevier B.V. All rights reserved.

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收藏

页码：96 / 100

页数：5

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