Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source

被引：49

作者：

Isobe, K

Watanabe, W

Matsunaga, S

Higashi, T

Fukui, K

Itoh, K

机构：

[1] Osaka Univ, Dept Mat & Life Sci, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS | 2005年 / 44卷 / 1-7期

关键词：

supercontinuum generation; photonic crystal fiber; two-photon fluorescence microscopy; ultrafast pulses; nonlinear optics;

D O I：

10.1143/JJAP.44.L167

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We report on a novel technique of multi-spectral two-photon-excited fluorescence microscopy by use of broadband supercontinuum light. A supercontinuum in the near-infrared region is generated in a 4.5-min-long photonic crystal fiber using a Ti:sapphire femtosecond oscillator. The supercontinuum is used as a multi-spectral excitation light source in a two-photon excited fluorescence microscope. We demonstrate that three-color fluorescence images of organelles in a cell can be simultaneously acquired.

引用

页码：L167 / L169

页数：3

共 15 条

[1]

Agrawal G., 2001, Nonlinear Fibers Optics, V3rd

[2]

ALFANO RR, 1989, SUPRECONTUNUUM LIGHT

[3] Spectral shaping of supercontinuum in a cobweb photonic-crystal fiber with sub-20-fs pulses [J].