Implication of Ile-69 and Thr-182 residues in kinetic characteristics of IRT-3 (TEM-32) beta-lactamase

被引：39

作者：

Farzaneh, S

Chaibi, EB

Peduzzi, J

Barthelemy, M

Labia, R

Blazquez, J

Baquero, F

机构：

[1] MNHN,CNRS,UMR 175,F-29000 QUIMPER,FRANCE

[2] MNHN,CNRS,URA 401,F-29000 QUIMPER,FRANCE

[3] HOSP RAMON Y CAJAL,E-28034 MADRID,SPAIN

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1996年 / 40卷 / 10期

关键词：

D O I：

10.1128/AAC.40.10.2434

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The substitution of a methionine for an isoleucine at position 69 (Met69IIe), which causes inhibitor resistance to TEM-type beta-lactamases (IRT-3 and IRT-I69), altered the positions of the Asn-170 and Glu-166 side chains as well as the position of the catalytic water molecule. A novel hydrogen bond between the hydroxyl of Thr-182 and the carbonyl of Glu-64 was expected to be responsible for the increase in the catalytic activity of the IST-T182 and IRT-3 enzymes compared with those of TEM-1 and IRT-I69, respectively.

引用

页码：2434 / 2436

页数：3

共 26 条

[1] A STANDARD NUMBERING SCHEME FOR THE CLASS-A BETA-LACTAMASES
AMBLER, RP
COULSON, AFW
FRERE, JM
GHUYSEN, JM
JORIS, B
FORSMAN, M
LEVESQUE, RC
TIRABY, G
WALEY, SG
[J]. BIOCHEMICAL JOURNAL, 1991, 276 : 269 - 270
[2] BARTHELEMY M, 1983, FEMS MICROBIOL LETT, V17, P151
[3] BELAAOUAJ A, 1994, FEMS MICROBIOL LETT, V120, P75
[4] BELAAOUAJ A, 1990, 10 INT M ANT CHEM SO
[5] CHARACTERIZATION OF A NEW TEM-TYPE BETA-LACTAMASE RESISTANT TO CLAVULANATE, SULBACTAM, AND TAZOBACTAM IN A CLINICAL ISOLATE OF ESCHERICHIA-COLI
BLAZQUEZ, J
BAQUERO, MR
CANTON, R
ALOS, I
BAQUERO, F
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (10) : 2059 - 2063
[6] BETA-LACTAMASE MUTATIONS FAR FROM THE ACTIVE-SITE INFLUENCE INHIBITOR BINDING
BONOMO, RA
DAWES, CG
KNOX, JR
SHLAES, DM
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1995, 1247 (01): : 121 - 125
[7] OHIO-1 BETA-LACTAMASE RESISTANT TO MECHANISM-BASED INACTIVATORS
BONOMO, RA
CURRIEMCCUMBER, C
SHLAES, DM
[J]. FEMS MICROBIOLOGY LETTERS, 1992, 92 (01) : 79 - 82
[8] COMPLEMENTARY ROLES OF MUTATIONS AT POSITION-69 AND POSITION-242 IN A CLASS-A BETA-LACTAMASE
BONOMO, RA
DAWES, CG
KNOX, JR
SHLAES, DM
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1995, 1247 (01): : 113 - 120
[9] CHARACTERIZATION AND AMINO-ACID-SEQUENCE OF IRT-4, A NOVEL TEM-TYPE ENZYME WITH A DECREASED SUSCEPTIBILITY TO BETA-LACTAMASE INHIBITORS
BRUN, T
PEDUZZI, J
CANICA, MM
PAUL, G
NEVOT, P
BARTHELEMY, M
LABIA, R
[J]. FEMS MICROBIOLOGY LETTERS, 1994, 120 (1-2) : 111 - 117
[10] DELAIRE M, 1992, J BIOL CHEM, V267, P20600

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