Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse

被引：133

作者：

Yamashita, T ^{[1
]}

Hige, T ^{[1
]}

Takahashi, T ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Neurophysiol, Tokyo 1130033, Japan

来源：

SCIENCE | 2005年 / 307卷 / 5706期

关键词：

D O I：

10.1126/science.1103631

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as "kiss-and-run" was unrelated to transmitter release. The release-related capacitance. change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine, 5'-triphosphate (GTP) analog GTPgammaS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.

引用

页码：124 / 127

页数：4

共 29 条

[1] Single synaptic vesicles fusing transiently and successively without loss of identity [J].

Aravanis, AM ;

Pyle, JL ;

Tsien, RW .

NATURE, 2003, 423 (6940) :643-647

[2] RAPID ENDOCYTOSIS COUPLED TO EXOCYTOSIS IN ADRENAL CHROMAFFIN CELLS INVOLVES CA2+, GTP, AND DYNAMIN BUT NOT CLATHRIN [J].

ARTALEJO, CR ;

HENLEY, JR ;

MCNIVEN, MA ;

PALFREY, CH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (18) :8328-8332

[3] Proteins involved in synaptic vesicle trafficking [J].

Augustine, GJ ;

Burns, ME ;

DeBello, WM ;

Hilfiker, S ;

Morgan, JR ;

Schweizer, FE ;

Tokumaru, H ;

Umayahara, K .

JOURNAL OF PHYSIOLOGY-LONDON, 1999, 520 (01) :33-41

[4]

BINZ T, 1994, J BIOL CHEM, V269, P1617

[5]

de Lange RPJ, 2003, J NEUROSCI, V23, P10164

[6]

Fesce Riccardo, 1994, Trends in Cell Biology, V4, P1, DOI 10.1016/0962-8924(94)90025-6

[7] Three modes of synaptic vesicular recycling revealed by single-vesicle imaging [J].

Gandhi, SP ;

Stevens, CF .

NATURE, 2003, 423 (6940) :607-613

[8] The SH3 domain of amphiphysin binds the proline-rich domain of dynamin at a single site that defines a new SH3 binding consensus sequence [J].

Grabs, D ;

Slepnev, VI ;

Zhou, SY ;

David, C ;

Lynch, M ;

Cantley, LC ;

DeCamilli, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (20) :13419-13425

[9] ATP is required at an early step in compensatory endocytosis in synaptic terminals [J].

Heidelberger, R .

JOURNAL OF NEUROSCIENCE, 2001, 21 (17) :6467-6474

[10] Imaging direct, dynamin-dependent recapture of fusing secretory granules on plasma membrane lawns from PC12 cells [J].

Holroyd, P ;

Lang, T ;

Wenzel, D ;

De Camilli, P ;

Jahn, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) :16806-16811

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