β1 integrin deficiency impairs migration and differentiation of mouse embryonic stem cell derived neurons

Cell-matrix interaction plays an important role during neuronal development, which is demonstrated by comparing wild type (D3)- and beta 1 integrin-deficient (G201) embryonic stem cell derived neurons. In D3 preparations complex networks of functionally coupled neurons with bi- and multipolar morphologies develop. In contrast, neuronal differentiation is retarded in G201 derived neurons, recognised by limited migration and restricted morphological differentiation. Furthermore, beta 1 integrin deficiency causes a delay in expression of major neurotransmitters like GABA and glutamate as well as of synaptophysin, These findings indicate a prominent role of beta 1 integrin for both morphological and chemical differentiation. (C) 1998 Elsevier Science Ireland Ltd. Ail rights reserved.