Gene sequences and specific detection for Panton-Valentine leukocidin

被引：63

作者：

Nakagawa, S ^{[1
]}

Taneike, I ^{[1
]}

Mimura, D ^{[1
]}

Iwakura, N ^{[1
]}

Nakayama, T ^{[1
]}

Emura, T ^{[1
]}

Kitatsuji, M ^{[1
]}

Fujimoto, A ^{[1
]}

Yamamoto, T ^{[1
]}

机构：

[1] Niigata Univ, Grad Sch Med & Dent Sci, Dept Infect Dis Control & Int Med, Div Bacteriol, Niigata, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 328卷 / 04期

关键词：

Panton-Valentine leukocidin genes; methicillin-resistant Staphylococcus aureus; community-acquired MRSA; Japanese isolate; real-time PCR; luk(PV)SF; mecA; spa;

D O I：

10.1016/j.bbrc.2005.01.054

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A new category of methicillin-resistant Staphlylococcus aureus (MRSA), called community-acquired MRSA (CA-MRSA), has emerged worldwide. In contrast to previous MRSA, most CA-MRSA carries the Panton-Valentine leukocidin (PVL) genes (luk(PV)SF) as a virulence genetic trait. Sequence analysis of the luk(PV)SF gene of a Japanese isolate demonstrated that the gene has more similarity to methicillin-susceptible S. aureus from France than MRSA from the United States. Based on the sequences, we developed a real-time PCR assay for the three key genes of CA-MRSA; that is, luk(PV)SF, mccA (for methicillin resistance), and spa (for S. aureus). Dual or triple assay for luk(PV)SF, mecA, and spa in one test tube became possible. The detection limit of the assay with probe and SYBR Green methods was between 2.7 and 2.7 x 10(1) CFU/ml. The assay detected PVL-positive MRSA in clinical (blood) isolates. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：995 / 1002

页数：8

共 26 条

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