Evaluation of a novel method based on PCR Restriction Fragment Length Polymorphism Analysis of the tuf gene for the identification of Staphylococcus species

被引：25

作者：

Kontos, F

Petinaki, E

Spiliopoulou, I

Maniati, M

Maniatis, AN

机构：

[1] Univ Thessalia, Sch Med, Dept Microbiol, Larisa, Greece

[2] Univ Patras, Sch Med, Dept Microbiol, GR-26110 Patras, Greece

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2003年 / 55卷 / 02期

关键词：

Staphylococcus species; tuf gene;

D O I：

10.1016/S0167-7012(03)00173-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method, based on PCR Restriction Fragment Length Polymorphism Analysis (PRA) of a part of the tuf gene (370 bp), was designed for the identification of 11 staphylococcal species, including the most common staphylococcal pathogens. A total of 258 clinical isolates were validated by this assay, and the results were in concordance with those obtained by the reference method of Kloos and Schleifer. (C) 2003 Elsevier B.V. All rights reserved.

引用

收藏

页码：465 / 469

页数：5

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