Localization of ASH1 mRNA particles in living yeast

被引：1192

作者：

Bertrand, E ^{[1
]}

Chartrand, P ^{[1
]}

Schaefer, M ^{[1
]}

Shenoy, SM ^{[1
]}

Singer, RH ^{[1
]}

Long, RM ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Dept Anat & Struct Biol & Cell Biol, Bronx, NY 10461 USA

来源：

MOLECULAR CELL | 1998年 / 2卷 / 04期

关键词：

D O I：

10.1016/S1097-2765(00)80143-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3'UTR (untranslated region) sequences. The SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation as well. Both She3myc and She1myc colocalized with the particle. Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization.

引用

页码：437 / 445

页数：9

共 48 条

[1] RELATIONSHIP OF ACTIN AND TUBULIN DISTRIBUTION TO BUD GROWTH IN WILD-TYPE AND MORPHOGENETIC-MUTANT SACCHAROMYCES-CEREVISIAE [J].

ADAMS, AEM ;

PRINGLE, JR .

JOURNAL OF CELL BIOLOGY, 1984, 98 (03) :934-945

[2] TRANSPORT AND LOCALIZATION OF EXOGENOUS MYELIN BASIC-PROTEIN MESSENGER-RNA MICROINJECTED INTO OLIGODENDROCYTES [J].

AINGER, K ;

AVOSSA, D ;

MORGAN, F ;

HILL, SJ ;

BARRY, C ;

BARBARESE, E ;

CARSON, JH .

JOURNAL OF CELL BIOLOGY, 1993, 123 (02) :431-441

[3] Mother and daughter are doing fine: Asymmetric cell division in yeast [J].

Amon, A .

CELL, 1996, 84 (05) :651-654

[4] High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A [J].

Ayscough, KR ;

Stryker, J ;

Pokala, N ;

Sanders, M ;

Crews, P ;

Drubin, DG .

JOURNAL OF CELL BIOLOGY, 1997, 137 (02) :399-416

[5] Actin: General principles from studies in yeast [J].

Ayscough, KR ;

Drubin, DG .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1996, 12 :129-160

[6]

Bassell GJ, 1998, J NEUROSCI, V18, P251

[7] ASSOCIATION OF POLY(A) MESSENGER-RNA WITH MICROTUBULES IN CULTURED NEURONS [J].

BASSELL, GJ ;

SINGER, RH ;

KOSIK, KS .

NEURON, 1994, 12 (03) :571-582

[8] Dimerization of the highly conserved light chain shared by dynein and myosin V [J].

Benashski, SE ;

Harrison, A ;

PatelKing, RS ;

King, SM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (33) :20929-20935

[9] Asymmetric accumulation of ASH1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells [J].

Bobola, N ;

Jansen, RP ;

Shin, TH ;

Nasmyth, K .

CELL, 1996, 84 (05) :699-709

[10]

Carson JH, 1997, CELL MOTIL CYTOSKEL, V38, P318, DOI 10.1002/(SICI)1097-0169(1997)38:4<318::AID-CM2>3.0.CO

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