Isolation and characterization of a Tritordeum cDNA encoding S-adenosylmethionine decarboxylase that is circadian-clock-regulated

被引:46
作者
Dresselhaus, T
Barcelo, P
Hagel, C
Lorz, H
Humbeck, K
机构
[1] UNIV COLOGNE,INST BOT,D-50931 COLOGNE,GERMANY
[2] INST ALLGEMEINE BOT,AMP 2,D-22609 HAMBURG,GERMANY
[3] AFRC,INST ARABLE CROPS RES,DEPT PHYSIOL & BIOCHEM,HARPENDEN AL5 2JQ,HERTS,ENGLAND
关键词
S-adenosylmethionine decarboxylase; circadian rhythm; ethylene; polyamines; Tritordeum; wounding;
D O I
10.1007/BF00020812
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sequence analysis of the two cDNA clones 47/11 and 50A which were isolated by differential screening of an explant cDNA library obtained from the monocot Tritordeum (hexaploid hybrid of diploid wild barley and tetraploid wheat lines) reveals that both clones include the same open reading frame (ORF). The sequence of this ORF shows a high degree of similarity with dicot S-adenosylmethionine decarboxylase (SAMDC) gene sequences and contains regions highly conserved in all known SAMDC sequences. It is further shown that the sequence represented by the cDNA clones 47/11 and 50A is derived from the wild barley (Hordeum chilense) genome, where it is present as a single-copy gene. Northern analyses indicate the corresponding transcript to accumulate in response to wounding and the transcript level changes with a circadian rhythm, having a peak in the middle of the light period. The periodicity continues in constant light, but is changed in constant darkness.
引用
收藏
页码:1021 / 1033
页数:13
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