The Clostridium botulinum GerAB germination protein is located in the inner membrane of spores

被引：16

作者：

Alberto, FO ^{[1
]}

Botella, L ^{[1
]}

Carlin, F ^{[1
]}

Nguyen-The, C ^{[1
]}

Broussolle, W ^{[1
]}

机构：

[1] INRA, UMR A408 Securite & Qual Prod Origine Vegetale, F-84914 Avignon, France

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 253卷 / 02期

关键词：

Clostridium botulinum; spore; germination; GerAB; membrane protein;

D O I：

10.1016/j.femsle.2005.09.037

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Clostridium botulinum dormant spores germinate in presence Of L-alanine via a specific receptor composed of GerAA, GerAB and GerAC proteins. In Bacillus subtilis spores, GerAA and GerAC proteins were located in the inner membrane of the spore. We studied the location of the GerAB protein in C. botulinum spore fractions by Western-blot analysis, using an antipeptidic antibody. The protein GerAB was in vitro translated and used to confirm the specificity of the antibodies. GerAB was not present in a coat and spore outer membrane fraction but was present in a fraction of decoated spores containing inner membrane. These results strongly suggest that the protein GerAB is located in the inner membrane of the spore. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：231 / 235

页数：5

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