Nonlinear microscopy

被引：11

作者：

Mertz, J ^{[1
]}

机构：

[1] INSERM EPI 00 02, Lab Neurophysiol & Nouvelles Microscopies, Paris, France

来源：

COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE IV PHYSIQUE ASTROPHYSIQUE | 2001年 / 2卷 / 08期

关键词：

non linear microscopy; two-photon fluorescence; second harmonic generation; optical biopsy; biological tissues;

D O I：

10.1016/S1296-2147(01)01260-4

中图分类号：

P1 [天文学];

学科分类号：

0704 ;

摘要：

We describe the basic principles of nonlinear optical microscopies based on two-photon excited fluorescence and on second-harmonic generation. Particular attention is given to the physical mechanisms underlying molecular second-harmonic generation, and the features unique to its signal contrast. We provide an overview of some applications of nonlinear microscopy including the, visualization of molecular 'flip-flop' dynamics in membranes, high-resolution measurements of inter-membrane separations, and high-sensitivity membrane potential imaging. Future developments of nonlinear microscopy are briefly considered. (C) 2001 Academic des sciences/Editions scientifiques et medicales Elsevier SAS.

引用

页码：1153 / 1160

页数：8

共 36 条

[1] Design of organic molecules with large two-photon absorption cross sections [J].

Albota, M ;

Beljonne, D ;

Brédas, JL ;

Ehrlich, JE ;

Fu, JY ;

Heikal, AA ;

Hess, SE ;

Kogej, T ;

Levin, MD ;

Marder, SR ;

McCord-Maughon, D ;

Perry, JW ;

Röckel, H ;

Rumi, M ;

Subramaniam, C ;

Webb, WW ;

Wu, XL ;

Xu, C .

SCIENCE, 1998, 281 (5383) :1653-1656

[2]

[Anonymous], 1997, T FLUOR SP

[3] PROBING MEMBRANE-POTENTIAL WITH NONLINEAR OPTICS [J].

BOUEVITCH, O ;

LEWIS, A ;

PINEVSKY, I ;

WUSKELL, JP ;

LOEW, LM .

BIOPHYSICAL JOURNAL, 1993, 65 (02) :672-679

[4]

Boyd R. W., 2003, NONLINEAR OPTICS

[5] Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery [J].

Brown, EB ;

Wu, ES ;

Zipfel, W ;

Webb, WW .

BIOPHYSICAL JOURNAL, 1999, 77 (05) :2837-2849

[6] Photolysis of caged calcium in femtoliter volumes using two-photon excitation [J].

Brown, EB ;

Shear, JB ;

Adams, SR ;

Tsien, RY ;

Webb, WW .

BIOPHYSICAL JOURNAL, 1999, 76 (01) :489-499

[7] High-resolution nonlinear optical imaging of live cells by second harmonic generation [J].

Campagnola, PJ ;

Wei, MD ;

Lewis, A ;

Loew, LM .

BIOPHYSICAL JOURNAL, 1999, 77 (06) :3341-3349

[8] Two-photon polymerization initiators for three-dimensional optical data storage and microfabrication [J].

Cumpston, BH ;

Ananthavel, SP ;

Barlow, S ;

Dyer, DL ;

Ehrlich, JE ;

Erskine, LL ;

Heikal, AA ;

Kuebler, SM ;

Lee, IYS ;

McCord-Maughon, D ;

Qin, JQ ;

Röckel, H ;

Rumi, M ;

Wu, XL ;

Marder, SR ;

Perry, JW .

NATURE, 1999, 398 (6722) :51-54

[9] 2-PHOTON SCANNING PHOTOCHEMICAL MICROSCOPY - MAPPING LIGAND-GATED ION-CHANNEL DISTRIBUTIONS [J].

DENK, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) :6629-6633

[10] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

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