Increase of lipid hydroperoxides in liver mitochondria and inhibition of cytochrome oxidase by carbon tetrachloride intoxication in rats

被引：17

作者：

Ikeda, K

Toda, M

Tanaka, K

Tokumaru, S

Kojo, S ^{[1
]}

机构：

[1] Nara Womens Univ, Dept Food Sci & Nutr, Nara 6308506, Japan

[2] Joetsu Univ Educ, Niigata 9438512, Japan

来源：

FREE RADICAL RESEARCH | 1998年 / 28卷 / 04期

关键词：

carbon tetrachloride; hydroperoxide; mitochondria; lipid peroxidation; cytochrome oxidase; haem a; necrosis;

D O I：

10.3109/10715769809070809

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cellular localization of lipid hydroperoxides was determined for the first time in mitochondria, microsomes and cytosol of rat liver using a specific method involving chemical derivatization and HPLC. Mitochondria contained the highest level of hydroperoxides. After 6 h of intragastric administration of carbon tetrachloride (CCl4) to rats (2 ml/kg body weight), the concentration of lipid hydroperoxides increased significantly in liver mitochondria and cytochrome oxidase activity was inhibited to 35% of the control rats. The mitochondrial content of haem a decreased to 60% of the control at 12 h of CCl4 administration. In vitro reaction of mitochondria with CCl4 caused inactivation of cytochrome oxidase. These observations suggested that cytochrome oxidase and haem a in mitochondria were targets of CCl4.

引用

页码：403 / 410

页数：8

共 37 条

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