The Kruppel-like zinc-finger gene ZNF236 is alternatively spliced and excluded as susceptibility gene for diabetic nephropathy

被引：8

作者：

Halama, N

Yard-Breedijk, A

Vardarli, I

Akkoyun, I

Yard, B

Janssen, B

van der Woude, FJ

机构：

[1] Heidelberg Univ, Med Dept 5, Heidelberg, Germany

[2] Heidelberg Univ, Eye Clin, Heidelberg, Germany

[3] Univ Hosp Mannheim, Mannheim, Germany

来源：

GENOMICS | 2003年 / 82卷 / 03期

关键词：

diabetic nephropathy; ZNF236; alternative splicing; zinc-finger; sequence analysis; chromosome; 18;

D O I：

10.1016/S0888-7543(03)00120-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Diabetic nephropathy (DN) is the most common cause of renal failure in the western hemisphere. Epidemiological studies have suggested a genetic susceptibility for DN. Linkage analysis showed evidence for a locus on chromosome 18q22.3-q23 in Turkish families. We report the construction of a transcript map of the target region on chromosome 18q22.3-q23 and analysis of the candidate gene ZNF236. By using recent publications, human genome databases, and a multitude of available protein-predicting programs, we obtained a detailed map of this 4.7-Mb-spanning region. We sequenced ZNF236 in patients with diabetic nephropathy and diabetes without nephropathy, as well as in unaffected controls. We observed multiple splice forms in all individuals but no mutation in any of the patients. It seems improbable, therefore, that ZNF236 is a gene that confers DN susceptibility. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：406 / 411

页数：6

共 16 条

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