Localization of chaperones DnaK and GroEL in bacterial inclusion bodies

被引：95

作者：

Carrió, MM

Villaverde, A ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Bellaterra 08193, Spain

[2] Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 10期

关键词：

D O I：

10.1128/JB.187.10.3599-3601.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By immunostaining and transmission electron microscopy, chaperones DnaK and GroEL have been identified at the solvent-exposed surface of bacterial inclusion bodies and entrapped within these aggregates, respectively. Functional implications of this distinct localization are discussed in the context of Escherichia coli protein quality control.

引用

页码：3599 / 3601

页数：3

共 20 条

[1] Ayling A, 1996, PROTEIN SCI, V5, P478
[2] Recombinant protein folding and misfolding in Escherichia coli
Baneyx, F
Mujacic, M
[J]. NATURE BIOTECHNOLOGY, 2004, 22 (11) : 1399 - 1408
[3] Distinct chaperone affinity to folding variants of homologous recombinant proteins
Boels, K
Carrió, MM
Arís, A
Corchero, JL
Villaverde, A
[J]. BIOTECHNOLOGY LETTERS, 1999, 21 (06) : 531 - 536
[4] Protein aggregation as bacterial inclusion bodies is reversible
Carrió, MM
Villaverde, A
[J]. FEBS LETTERS, 2001, 489 (01) : 29 - 33
[5] Proteolytic digestion of bacterial inclusion body proteins during dynamic transition between soluble and insoluble forms
Carrió, MM
Corchero, JL
Villaverde, A
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1999, 1434 (01): : 170 - 176
[6] Role of molecular chaperones in inclusion body formation
Carrió, MM
Villaverde, A
[J]. FEBS LETTERS, 2003, 537 (1-3) : 215 - 221
[7] Construction and deconstruction of bacterial inclusion bodies
Carrió, MM
Villaverde, A
[J]. JOURNAL OF BIOTECHNOLOGY, 2002, 96 (01) : 3 - 12
[8] Dynamics of in vivo protein aggregation:: building inclusion bodies in recombinant bacteria
Carrió, MM
Corchero, JL
Villaverde, A
[J]. FEMS MICROBIOLOGY LETTERS, 1998, 169 (01) : 9 - 15
[9] GroEL/GroES-mediated folding of a protein too large to be encapsulated
Chaudhuri, TK
Farr, GW
Fenton, WA
Rospert, S
Horwich, AL
[J]. CELL, 2001, 107 (02) : 235 - 246
[10] Limited in vivo proteolysis of aggregated proteins
Corchero, JL
Cubarsi, R
Enfors, SO
Villaverde, A
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 237 (02) : 325 - 330

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