Absence of follicle-stimulating hormone receptor activating mutations in women with iatrogenic ovarian hyperstimulation syndrome

被引:34
作者
d'Alva, CB
Serafini, P
Motta, E
Kohek, MBD
Latronico, AC
Mendonca, BB
机构
[1] Univ Sao Paulo, Hosp Clin, Lab Hormonios & Genet Mol, Unidade Endocrinol Desenvolvimento, BR-05403900 Sao Paulo, SP, Brazil
[2] Huntington Ctr Med Reprod, Sao Paulo, SP, Brazil
[3] Fdn Fac Ciencias Med Porto Alegre, Dept Ciencias Fisiol, Porto Alegre, RS, Brazil
基金
巴西圣保罗研究基金会;
关键词
FSH receptor; ovarian hyperstimulation syndrome; polymorphisms;
D O I
10.1016/j.fertnstert.2004.12.044
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To analyze the FSH receptor gene in women with iatrogenic ovarian hyperstimulation syndrome (OHSS). Design: Clinical and molecular studies. Setting: University hospital and private clinic. Patient(S): Twenty-nine women who developed moderate or severe OHSS after ovulation induction for IVF. In addition, 50 fertile normal women were used as controls. Intervention(s): Peripheral blood was used for DNA extraction. The exons 4, 7, 9, and 10 of the FSH receptor gene were amplified by polymerase chain reaction (PCR) followed by automatic direct sequencing. Main Outcome Measure(s): Hormonal results and automatic sequencing analysis. Result(s): Thirteen patients developed moderate OHSS and 16 patients developed the severe form of the syndrome. Automatic sequencing revealed no activating mutations in all patients studied. We found two known polymorphisms in linkage disequilibrium, Ala307Thr and Ser680Asn, in 79.3% of the patients (44.8% in hetrozygous and 34.5% in homozygous state). These polymorphisms were found with similar frequency in the 50 normal fertile women, Conclusion(s): We conclude that the FSH receptor genotype did not play a significant role in the risk of iatrogenic OHSS in this cohort. (c) 2005 by American Society for Reproductive Medicine.
引用
收藏
页码:1695 / 1699
页数:5
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