Molecular mechanism of YbtA-mediated transcriptional regulation of divergent overlapping promoters ybtA and irp6 of Yersinia enterocolitica

被引：21

作者：

Anisimov, R ^{[1
]}

Brem, D ^{[1
]}

Heesemann, E ^{[1
]}

Rakin, A ^{[1
]}

机构：

[1] Max Von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 250卷 / 01期

关键词：

Yersiniae; YbtA binding sites; gene regulation; overlapping promoters;

D O I：

10.1016/j.femsle.2005.06.040

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genes of Yersiniae spp. involved in production of the siderophore yersiniabactin are located on the high-pathogenicity island (HPI). Their transcription is controlled by the AraC/XiIS-like transcriptional regulator YbtA encoded within the HP1. YbtA-regulated divergent and overlapping ybtA and irp6 promoters contain three YbtA binding sites, RS1, RS2 and RS3. Deleting RSs systematically and using ybtA and irp6 transcriptional fusions, we determined that different modes of YbtA binding are responsible for activation of irp6 and repression of ybtA. Based on these data, we propose a model of irp6 and ybtA promoter regulation. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：27 / 32

页数：6

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