Production of thermostable lipolytic activity by Thermus species

被引:31
作者
Fuciños, P
Domínguez, A
Sanromán, MA
Longo, MA
Rúa, ML
Pastrana, L
机构
[1] Univ Vigo, Dept Biochem Genet & Immunol, As Lagoas 32004, Ourense, Spain
[2] Univ Vigo, Dept Chem Engn, Vigo 36310, Spain
关键词
D O I
10.1021/bp050080g
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A quantitative screening for intra- and extracellular lipolytic activity was performed in submerged cultures of four Thermus strains using two different media (named T or D medium). Major differences in the extracellular lipolytic activity were observed in T medium, the highest values being for Thermus thermophilus HB27 and Thermus aquaticus YT1 strains (18 and 33 U/L, respectively). Two enzymes with lipase/esterase activity were identified in the four Thermus strains by zymogram. analysis, with molecular weights of 34 and 62 kDa. No kinetic typification of the enzymes as primary metabolites was possible for any of the Thermus strains, because of the lack of a good fitting of the experimental lipolytic activity production rates to the Luedecking and Piret model. However, a linear relationship was found between the absolute values of biomass and total lipase/esterase activity (sum of intracellular and extracellular). For T. thermophilus HB27, an increase in the aeration rate caused the increase in the production of biomass and, particularly, intracellular lipolytic activity but the extracellular lipolytic activity was not affected except for the series with the strongest oxygen limitation. Transmission electronic microscopy revealed that T. thermophilus HB27 formed rotund bodies surrounded by a common membrane in cultures in the early stationary phase. The results suggest the occurrence of a specific mechanism of lipase/esterase secretion that might be due to the different composition and permeability of the cell membranes and those surrounding the rotund bodies.
引用
收藏
页码:1198 / 1205
页数:8
相关论文
共 41 条
[1]   Determination of the kinetic parameters during continuous cultivation of the lipase producing thermophile Bacillus sp. IHI-91 on olive oil [J].
Becker, P ;
AbuReesh, I ;
Markossian, S ;
Antranikian, G ;
Markl, H .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1997, 48 (02) :184-190
[2]  
BECKER RJ, 1984, MICROBIOS, V41, P115
[3]   Response of the thermophile Thermus sp RQ-1 to hyperbaric air in batch and fed-batch cultivation [J].
Belo, I ;
Pinheiro, R ;
Mota, M .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2000, 53 (05) :517-524
[4]  
Berger JL, 1995, APPL MICROBIOL BIOT, V44, P81, DOI [10.1007/s002530050523, 10.1007/BF00164484]
[5]   Purification, properties and characterization of a high-molecular-mass beta-galactosidase isoenzyme from Thermus aquaticus YT-I [J].
Berger, JL ;
Lee, BH ;
Lacroix, C .
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1997, 25 :29-41
[6]   THERMUS AQUATICUS GEN N AND SP N A NONSPORULATING EXTREME THERMOPHILE [J].
BROCK, TD ;
FREEZE, H .
JOURNAL OF BACTERIOLOGY, 1969, 98 (01) :289-&
[7]  
BROCK TD, 1981, PROKARYOTES HDB HABI, P978
[8]   The periplasmic space in Thermus thermophilus:: evidence from a regulation-defective S-layer mutant overexpressing an alkaline phosphatase [J].
Castán, P ;
Zafra, O ;
Moreno, R ;
de Pedro, M ;
Vallés, C ;
Cava, F ;
Caro, E ;
Schwarz, H ;
Berenguer, J .
EXTREMOPHILES, 2002, 6 (03) :225-232
[9]   THERMOPHILIC BLUE-GREEN ALGAE AND THERMAL ENVIRONMENT [J].
CASTENHOLZ, RW .
BACTERIOLOGICAL REVIEWS, 1969, 33 (04) :476-+
[10]  
Cowan D A, 1992, Biochem Soc Symp, V58, P149