Direct development of functionally mature tryptase/chymase double-positive connective tissue-type mast cells from primate embryonic stem cells

被引：16

作者：

Ma, Feng ^{[1
,3
]}

Kambe, Naotomo ^{[6
]}

Wang, Dan ^{[3
]}

Shinoda, Gen ^{[1
]}

Fujino, Hisanori ^{[1
]}

Umeda, Katsutsugu ^{[1
]}

Fujisawa, Akihiro ^{[2
]}

Ma, Lina ^{[1
]}

Suemori, Hirofumi ^{[4
]}

Nakatsuji, Norio ^{[4
]}

Miyachi, Yoshiki ^{[2
]}

Torii, Ryuzo ^{[5
]}

Tsuji, Kohichiro ^{[3
]}

Heike, Toshio ^{[1
]}

Nakahataa, Tatsutoshi ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Pediat, Sakyo Ku, Kyoto 6068507, Japan

[2] Kyoto Univ, Grad Sch Med, Dept Dermatol, Kyoto, Japan

[3] Univ Tokyo, Inst Med Sci, Div Cellular Therapy, Tokyo, Japan

[4] Kyoto Univ, Res Ctr Regenerat Med, Kyoto, Japan

[5] Shiga Univ Med Sci, Res Ctr Anim Llife Sci, Otsu, Shiga, Japan

[6] Chiba Univ, Grad Sch Med, Dept Dermatol, Chiba, Japan

来源：

STEM CELLS | 2008年 / 26卷 / 03期

关键词：

embryonic stem cells; mast cells; primate; development; chymase; tryptase;

D O I：

10.1634/stemcells.2007-0348

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Conditions that influence the selective development or recruitment of connective tissue-type and mucosal-type mast cells (MCs) are not well understood. Here, we report that cynomolgus monkey embryonic stem (ES) cells cocultured with the murine aorta-gonad-mesonephros-derived stromal cell line AGM-S1 differentiated into cobblestone (CS)-like cells by day 10-15. When replated onto fresh AGM-S1 with the addition of stem cell factor, interleukin-6, and Flt3 ligand, these CS-like cells displayed robust growth and generated almost 100% tryptase/chymase double-positive MCs within 3 weeks. At all time points, the percentage of tryptase-positive cells did not exceed that of chymase-positive cells. These ES-derived MCs were CD45+/Kit+/CD31+/CD203c+/HLA-DR- and coexpressed a high-affinity IgE receptor on their surface, which was upregulated after IgE exposure. Electron microscopy showed that they contained many electron dense granules. Moreover, ES-derived MCs responded to stimulation by via IgE and substance P by releasing histamine. These results indicate that ES-derived MCs have the phenotype of functionally mature connective tissue-type MCs. The rapid maturation of ES-derived MCs suggests a unique embryonic pathway in primates for early development of connective tissue-type MCs, which may be independent from the developmental pathway of mucosal-type MCs.

引用

页码：706 / 714

页数：9

共 26 条

[1] DEVELOPMENT OF HUMAN MAST-CELLS INVITRO [J].

FURITSU, T ;

SAITO, H ;

DVORAK, AM ;

SCHWARTZ, LB ;

IRANI, AMA ;

BURDICK, JF ;

ISHIZAKA, K ;

ISHIZAKA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) :10039-10043

[2] Electroporation of cynomolgus monkey embryonic stem cells [J].

Furuya, M ;

Yasuchika, K ;

Mizutani, K ;

Yoshimura, Y ;

Nakatsuji, N ;

Suemori, H .

GENESIS, 2003, 37 (04) :180-187

[3] Gene transfer into nonhuman primate hematopoietic stem cells: Implications for gene therapy [J].

Hanazono, Y ;

Terao, K ;

Ozawa, K .

STEM CELLS, 2001, 19 (01) :12-23

[4]

Igarashi Y, 1996, CLIN EXP ALLERGY, V26, P597

[5] 2 TYPES OF HUMAN MAST-CELLS THAT HAVE DISTINCT NEUTRAL PROTEASE COMPOSITIONS [J].

IRANI, AA ;

SCHECHTER, NM ;

CRAIG, SS ;

DEBLOIS, G ;

SCHWARTZ, LB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (12) :4464-4468

[6]

IRANI AA, 1992, IMMUNOLOGY, V77, P136

[7]

IRANI AMA, 1987, J IMMUNOL, V138, P4381

[8] Human skin-derived mast cells can proliferate while retaining their characteristic functional and protease phenotypes [J].

Kambe, N ;

Kambe, M ;

Kochan, JP ;

Schwartz, LB .

BLOOD, 2001, 97 (07) :2045-2052

[9] Interleukin-6 directly modulates stem cell factor-dependent development of human mast cells derived from CD34+ cord blood cells [J].

Kinoshita, T ;

Sawai, N ;

Hidaka, E ;

Yamashita, T ;

Koike, K .

BLOOD, 1999, 94 (02) :496-508

[10]

KIRSHENBAUM AS, 1991, J IMMUNOL, V146, P1410

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