Forced expression of the Oct-4 gene influences differentiation of embryonic stem cells

被引：2

作者：

Du, ZW ^{[1
]}

Cong, XQ ^{[1
]}

Yao, Z ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2001年 / 46卷 / 17期

基金：

中国国家自然科学基金;

关键词：

Oct-4; gene; embryonic stem cell; differentiation;

D O I：

10.1007/BF03187028

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

By transfecting an Oct-4 expression plasmid Into embryonic stem cells (ES cells), the ES-O cell line was constructed, which sustained the expression of Oct-4 gene when induced by retinoic acid. Forced expression of Oct-4 gene could not sustain the stem property of ES-O cells without the differentiation inhibiting factor LIF, but if LIF exists, forced expression of Oct-4 gene could enhance the ability to sustain the undifferentiation state and Inhibit cell differentiation induced by retinoic acid. It was Indicated that Oct-4 must cooperate with LIF to sustain the undifferentiation state of ES cells. During the cell differentiation, ES-O cells tend to differentiate Into neural cells, suggesting that forced expression of Oct-4 gene may be In relation with the differentiation of neuroderm.

引用

页码：1446 / 1449

页数：4

共 20 条

[1] Oct-4: more than just a POUerful marker of the mammalian germline? [J].