Detection of human metapneumovirus RNA sequences in nasopharyngeal aspirates of young French children with acute bronchiolitis by real-time reverse transcriptase PCR and phylogenetic analysis

被引:50
作者
Bouscambert-Duchamp, M
Lina, B
Trompette, A
Moret, H
Motte, J
Andréoletti, L
机构
[1] Hop Robert Debre, Microbiol Serv, Virol Lab, CHU & ER 3798,IFR 53,Fac Med Reims, F-51092 Reims, France
[2] Hospices Civils Lyon, Virol Lab, Lyon, France
[3] Fac Med Reims, IFR 53, Serv Urgences Medico Chirurg Pediat, Champagne Ardenne, France
[4] Fac Med Reims, IFR 53, ER 3798, Champagne Ardenne, France
关键词
D O I
10.1128/JCM.43.3.1411-1414.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human metapneumovirus (HMPV) was the unique viral pathogen detected by a real-time reverse transcriptase PCR (RT-PCR) assay in 6 (6.4%) of 94 consecutive French children hospitalized for acute bronchiolitis from September 2001 to June 2002. This virus was identified as the third etiological cause of bronchiolitis, after respiratory syncytial virus and rhinovirus (35 [37%] and 21 [22%] of 94 cases, respectively). Phylogenetic analysis of F-gene sequences demonstrated the cocirculation of distinct HMPV genotypes during this study. These findings highlight the need to implement a rapid HMPV RT-PCR detection assay for the clinical diagnosis of respiratory infections in pediatric patients with bronchiolitis.
引用
收藏
页码:1411 / 1414
页数:4
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