Mutually exclusive expression of odorant receptor transgenes

被引：192

作者：

Serizawa, S

Ishii, T

Nakatani, H

Tsuboi, A

Nagawa, F

Asano, M

Sudo, K

Sakagami, J

Sakano, H ^{[1
]}

Ijiri, T

Matsuda, Y

Suzuki, M

Yamamori, T

Iwakura, Y

Sakano, H ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Sci, Dept Biophys & Biochem, Tokyo 1130032, Japan

[2] Univ Tokyo, Inst Med Sci, Ctr Med Expt, Tokyo 1088639, Japan

[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[4] Hokkaido Univ, Grad Sch Environm Earth Sci, Div Biosci, Lab Cytogenet, Sapporo, Hokkaido 0600810, Japan

[5] Kumamoto Univ, Ctr Anim Resources & Dev, Kumamoto 8620976, Japan

[6] Natl Inst Basic Biol, Div Speciat Mech 1, Okazaki, Aichi 4448585, Japan

来源：

NATURE NEUROSCIENCE | 2000年 / 3卷 / 07期

关键词：

D O I：

10.1038/76641

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To study the mutually exclusive expression of odorant receptor (OW) genes, we generated transgenic mice that carried the murine OR gene MOR28. Expression of the transgene and the endogenous MOR28 was distinguished by using two different markers, beta -galactosidase and green fluorescent protein (GFP), respectively. Double staining of the olfactory epithelium revealed that the two genes were rarely expressed simultaneously in individual olfactory neurons. A similar exclusion was also observed between differently tagged but identical transgenes integrated into the same locus of one particular chromosome. Although allelic inactivation has been reported for the choice between the maternal and paternal alleles, this is the first demonstration of mutually exclusive activation among non-allelic OR gene members with identical coding and regulatory sequences. Such an unusual mode of gene expression, monoallelic and mutually exclusive, has previously been shown only for the antigen-receptor genes of the immune system.

引用

页码：687 / 693

页数：7

共 34 条

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