Thin Multilayer Films and Microcapsules Containing DNA Quadruplex Motifs

被引：9

作者：

Cavalieri, Francesca ^{[2
]}

Ng, Sher Leen ^{[1
]}

Mazzuca, Claudia ^{[2
]}

Jia, Zhongfan ^{[3
]}

Bulmus, Volga ^{[3
]}

Davis, Thomas P. ^{[3
]}

Caruso, Frank ^{[1
]}

机构：

[1] Univ Melbourne, Dept Chem & Biomol Engn, Ctr Nanosci & Nanotechnol, Parkville, Vic 3010, Australia

[2] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00173 Rome, Italy

[3] Univ New S Wales, Sch Chem Engn, Ctr Adv Macromol Design, Sydney, NSW 2052, Australia

来源：

SMALL | 2011年 / 7卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

DUAL-POLARIZATION INTERFEROMETRY; CIRCULAR-DICHROISM; BLOCK-COPOLYMERS; NUCLEIC-ACIDS; FRAYED WIRES; REAL-TIME; PORPHYRIN; PROTEIN; BINDING; RAFT;

D O I：

10.1002/smll.201001246

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The assembly of multifunctional nanostructures bearing G-quadruplex motifs broadens the prospects of using G-quadruplexes as therapeutic carriers. Herein, we report the synthesis and characterization of an oligodeoxyguanosine, G15-mer polymer conjugate. We demonstrate that G15-mer oligonucleotides grafted to a polymer chain preserve the ability to self-assemble into ordered structures. The G-quadruplex-polymer conjugates were assembled onto a surface via hybridization with 30-mer cytosine strands, C30-mer, using a layer-by-layer approach to form microcapsules. A mechanism for the sequential assembly of the multilayer films and microcapsules is presented. We further investigate the photophysical behavior of porphyrin TMPyP4 bound to multilayer-coated particles. This study shows that the multilayer films bear residual and functional quadruplex moieties that can be used to effectively bind therapeutic agents.

引用

页码：101 / 111

页数：11

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