Direct spectroscopic evidence for a high-spin Fe(IV) intermediate in tyrosine hydroxylase

被引：143

作者：

Eser, Bekir E.

Barr, Eric W.

Frantorn, Patrick A.

Saleh, Lana

Bollinger, J. Martin, Jr. ^{[1
]}

Krebs, Carsten

Fitzpatrick, Paul F.

机构：

[1] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA

[2] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA

[3] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA

[4] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 37期

关键词：

D O I：

10.1021/ja074446s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tyrosine hydroxylase, a member of the aromatic amino acid hydroxylase family, uses a mononuclear Fe(H) and tetrahydropterin for hydroxylation of tyrosine to dihydroxyphenylalanine. Rapid-freeze quench Mossbauer spectroscopy has now provided direct evidence for the presence of an Fe(IV) intermediate in the reaction catalyzed by tyrosine hydroxylase. Rapid-quench techniques provide support for the kinetic competence of this species as the hydroxylating intermediate. This is the first direct evidence for a mononuclear Fe(IV) intermediate in an enzymatic aromatic hydroxylation reaction.

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页码：11334 / +

页数：3

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