B3-adrenergic receptor gene polymorphism is not associated with hypertension in NIDDM patients without nephropathy

Objective: We studied the relationship between a Trp64Arg mutation of beta(3)-adrenergic receptor gene and hypertension in Japanese NIDDM patients without nephropathy. Research Design and Methods: Eighty-three Japanese NIDDM patients (46 men, 37 women, known duration of 12 [5 -31], age of 55 [40-69] years, median and [range]) with normoalbuminuria and microalbuminuria were studied. The Trp64Arg mutation in the beta(3)-adrenergic receptor gene was determined by the PCR-RFLP method. Results: The frequency of the mutated allele was not greater in the hypertensive patients (18.2%, n = 37) than in the normotensive (20.2%, n=46) patients. Genotypes with a mutate allele (i.e. Trp64/Arg64+Arg64/Arg64) were observed in 23.5% in the hypertensive patients and in 35.7% in the normotensive patients, respectively. A similar finding in the allele frequency was observed when the analysis was made separately in the normoalbuminuric and microalbuminuric patients, respectively. Conclusion: These results suggest that a Trp64Arg mutation in the beta(3)-adrenergic receptor gene is not associated with hypertension in Japanese NIDDM patients without nephropathy.