A recombinant single-chain antibody fragment that neutralizes toxin II from the venom of the scorpion Androctonus australis hector

被引：52

作者：

Mousli, M

Devaux, C

Rochat, H

Goyffon, M

Billiald, P

机构：

[1] Fac Med Nord, Ingn Prot Lab, CNRS, UMR 6560, F-13916 Marseille 20, France

[2] Museum Natl Hist Nat, Lerai, F-75231 Paris 05, France

[3] Fac Pharm, F-37200 Tours, France

来源：

FEBS LETTERS | 1999年 / 442卷 / 2-3期

关键词：

neurotoxin; single-chain antibody fragment; single-chain antibody; neutralization; scorpion; Androctonus australis hector;

D O I：

10.1016/S0014-5793(98)01647-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibody 4C1 specifically binds to and neutralizes the most potent neurotoxin (AahII) of the scorpion Androctonus nustralis. The cDNAs encoding the variable regions of this antibody were isolated by PCR-mediated cloning. A single-chain Fv gene was engineered and expressed in Escherichia coli, The recombinant protein had neutralizing activity similar to that of the intact antibody in vitro and in vivo. We have thus neutralized the pharmacological and biological properties of a scorpion neurotoxin with a single-chain Fv, which opens nem perspectives for the treatment of envenomizations, (C) 1999 Federation of European Biochemical Societies.

引用

页码：183 / 188

页数：6

共 41 条

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