Structure of the O-specific polysaccharide of Proteus penneri strain 25 containing N-(L-alanyl) and multiple O-acetyl groups in a tetrasaccharide repeating unit

Based on sugar and methylation analyses, O-deacetylation, Smith degradation, and H-1 and C-13 NMR spectroscopy, including 2D COSY, H-1-detected H-1, C-13 heteronuclear single-quantum coherence (HSQC), and H-1-detected H-1, C-13 heteronuclear multiple-bond connectivity (HMBC) experiments, the following structure of the O-specific polysaccharide of Proteus penneri strain 25 was established: [GRAPHICS] where D-GlcN(L-Ala) is 2-(L-alanylamido)-2-deoxy-D-glucose. (C) 1997 Elsevier Science Ltd.