Effect of chronic electrostimulation of rabbit skeletal muscle on calmodulin level and protein kinase activity

被引：16

作者：

Antipenko, A ^{[1
]}

Frías, JA ^{[1
]}

Parra, J ^{[1
]}

Cadefau, JA ^{[1
]}

Cussó, R ^{[1
]}

机构：

[1] Univ Barcelona, Fac Med, Inst Invest Biomed August Pi I Sunyer, Dept Ciencias Fisiol 1,Unitat Bioquim, Barcelona, Spain

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 1999年 / 31卷 / 02期

关键词：

muscle electrostimulation; protein kinases; calmodulin;

D O I：

10.1016/S1357-2725(98)00112-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(a) Chronic electrostimulation of fast-twitch skeletal muscles makes them resemble slow-twitch muscles. The involvement of second-messenger cascades in this muscle reprogramming is not well understood. The goal of this study was to examine protein kinase activities and calmodulin levels as a function of the duration of electrostimulation, (b) Fast-twitch rabbit muscle was subjected to continuous low-frequency electrostimulation for 2 weeks, The extensor digitorum longus was taken and examined for calmodulin concentration and cAMP-dependent (PKA), Ca2+-phospholipid-dependent (PKC) and Ca2+-calmodulin-dependent (CaM kinase or PKB) protein kinase activities. (c) Electrostimulation for 14 days led to a significant increase in total calmodulin level and PKB activity, both rising in the cytosolic fraction. Protein kinase C translocated to the membrane fraction, although total activity did not change. (d) These changes could be related with electrostimulation-induced changes in excitation-contraction coupling. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：303 / 310

页数：8

共 36 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] CA-ATPASE ISOZYME EXPRESSION IN SARCOPLASMIC-RETICULUM IS ALTERED BY CHRONIC STIMULATION OF SKELETAL-MUSCLE [J].