Quercetin-induced apoptosis involves increased hTERT enzyme activity of leukemic cells

被引：30

作者：

Avci, Cigir Biray ^{[2
]}

Yilmaz, Sunde ^{[2
]}

Dogan, Zeynep Ozlem ^{[2
]}

Saydam, Guray ^{[3
]}

Dodurga, Yavuz ^{[4
]}

Ekiz, Huseyin Atakan ^{[1
]}

Kartal, Melis ^{[1
]}

Sahin, Fahri ^{[3
]}

Baran, Yusuf ^{[1
]}

Gunduz, Cumhur ^{[2
]}

机构：

[1] Izmir Inst Technol, Dept Mol Biol & Genet, Fac Sci, TR-35430 Izmir, Turkey

[2] Ege Univ, Sch Med, Dept Med Biol, Izmir, Turkey

[3] Ege Univ, Sch Med, Dept Hematol, Izmir, Turkey

[4] Pamukkale Univ, Sch Med, Dept Med Biol, Denizli, Turkey

来源：

HEMATOLOGY | 2011年 / 16卷 / 05期

关键词：

Quercetin; hTERT; Telomerase; Apoptosis; Leukemia; ACUTE LYMPHOBLASTIC-LEUKEMIA; CORONARY-HEART-DISEASE; DIETARY FLAVONOIDS; MYELOID-LEUKEMIA; CANCER; RISK; ARABIDOPSIS; VITRO;

D O I：

10.1179/102453311X13085644680104

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We aimed to examine the growth suppressive effects of quercetin on acute promyelocytic and lymphoblastic leukemia and chronic myeloid leukemia, and to find out whether the growth suppression is related to the blocking of telomerase enzyme activity. Cytotoxic effects of quercetin were shown by trypan blue analyses. Apoptotic effects of quercetin were examined by acridine orange and ethidium bromide staining by fluorescence microscopy. The effects of quercetin on telomerase enzyme activity were shown by hTERT Quantification Kit. Our results demonstrated that quercetin has antiproliferative and apoptotic effects on T-cell acute lymphoblastic leukemia (ALL), acute promyelocytic leukemia, and chronic myeloid leukemia (CML) cells. We also showed for the first time by this study that quercetin suppresses the activity of telomerase in ALL and CML cells. The results of this study show the importance of quercetin for its therapeutic potential in treatment of leukemias.

引用

页码：303 / 307

页数：5

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