In vitro techniques for studying drug metabolism

被引：79

作者：

Guengerich, FP ^{[1
]}

机构：

[1] VANDERBILT UNIV, CTR MOL TOXICOL, SCH MED, NASHVILLE, TN 37232 USA

来源：

JOURNAL OF PHARMACOKINETICS AND BIOPHARMACEUTICS | 1996年 / 24卷 / 05期

关键词：

drug metabolism; in vitro methods; cytochrome P450; glutathione S-transferase; enzymology; antibodies; inhibitors; recombinant DNA technology;

D O I：

10.1007/BF02353478

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A considerable amount of information has been obtained about human enzymes involved in drug metabolism. Cytochrome P450 is presented as a paradigm in order to illustrate the experimental techniques now available. In vitro assays can be done with tissue slices, microsomes, or even short-term cell cultures. In addition, recombinant enzymes can be produced in a variety of different vector systems. Information about catalytic selectivity regarding new drugs can be obtained, as wed as important information about potential drug-drug interactions and bioavailability. Such studies play a role in the drug development process as well as metabolism and safety assessment.

引用

页码：521 / 533

页数：13

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